This study was conducted to determine the effect of pentoxifylline levels on sperm motility, survival rate, sperm membrane integrity of frozen semen and fresh-extended equine semen in Jeju cross-bred horses. As a result of sperm characteristic comparison depending on pentoxifylline levles at 30 minutes post-thaw, the progressive motilities were 53.25±2.87(4mM pentoxifylline) and 50.28±2.14(8mM pentoxifylline) and significantly higher compared to the control group(40.09±5.15) and other treatment group(16mM pentoxifylline, 41.27±2.82). The progressive fast motility were 22.44±1.62(4mM pentoxifylline,) and 22.74±3.07(8mM pentoxifylline) and significantly higher compared to the control group(13.47±1.48) and other treatment group(16mM pentoxifylline, 14.66±3.68)(p<0.05). As a result of sperm characteristic comparison depending on pentoxifylline levles at 30 minutes post-thaw were 68.96±1.64(4mM pentoxifylline) and 67.90±6.72(8mM pentoxifylline) and significantly higher compared to the control group(53.48±4.84) and other treatment group(16mM pentoxifylline, 58.14±2.65).(p<0.05) In conclusion, these results suggest that treatment groups with 4mM and 8mM pentoxifylline were higher compare to equine seperm mobility and the control group and treatment groups with more than 16mM pentoxifylline has a negative effect on sperm characteristics. After thawing, the total motility in post-thawed equine sperm has increased by 10 percent for 1 hour. these results suggest that pentoxifylline contributes to the improvement of the equine sperm motility and characteristics in post-thawed semen.