Female patient age is the key factor determining IVF success. Therefore, culture media that improve either embryo quality or yield for older patients undergoing IVF would be a significant advance for infertility treatment. Nicotinamide adenine dinucleotide (NAD+) is central to the regulation of ageing. We have shown that treatment of aged female mice with the NAD+ precursor nicotinamide mononucleotide (NMN) improves fertility. This study aimed to assess the effect of supplementing embryo culture media with NMN on mouse embryo development. Mature oocytes were collected from the ampulla of aged (11–12 months) or young (4 weeks) female C57Bl6 mice primed with PMSG and hCG. Following IVF presumptive zygotes were randomly allocated to culture droplets supplemented with 0,0.1,1 or 10µM NMN, and cultured for 6 days. After 5 days there were significantly more blastocysts cultured with 1µM NMN (86.9 ± 5.6%) compared to controls (66.1 ± 7.3%;P<0.05), and likewise with 1µM (93.5 ± 4.9%) and 10µM NMN (89.3 ± 1.6%) compared to controls (60.7 ± 6.7%;P<0.01) on day 6. When the same experiment was repeated using 4-week-old mice, there was no effect of NMN on any developmental readouts. To assess embryo quality embryos from aged females were cultured in either 0µM or 1µM NMN, and total cell number and the ratio of inner cell mass (ICM) to trophectoderm (TE) were assessed on day 6. Embryos from young females were used as positive controls. Embryos from young mice (93.1 ± 5.9) and aged embryos treated with NMN (91.0 ± 4.23) had significantly more cells than untreated aged embryos (72.83 ± 4.1;P=0.01). There were no differences in the ratio of ICM:TE between groups. These results suggest that embryos of aged mice are deficient in NAD+ signalling and support the hypothesis that supplementation with NAD+ precursors can ameliorate the effects of ageing on fertility.