Calcium is important for physiological functioning in many tissues and is essential in mucus secretion. Previously reported, Mucin secretion is regulated predominantly by cytosolic calcium-dependent pathways. Cytosolic of calcium are regulated by calcium channels such as TRPV6, NCX1, and PMCA1. A549 cell line was treated with 10-8 M dexamethasone (DEX) and 10-6 M RU486. Subsequently, the expression of TRPV6, NCX1, and PMCA1 in A549 cell line were examined. There was no significant differences in PMCA1 expressions in DEX-treated groups, but TRPV6 was increased in DEX-treated groups and was recovered by DEX+RU486 treatment. NCX1 was decreased in DEX-treated groups and was recovered by RU486 treatment. In addition, mucin secretion, related genes MUC4 and MUC5AC, was also decreased by DEX treatment. Control of calcium channel gene expression may affect the control of mucus secretion in the lung cancer. These results could be used for understanding the basis of treatment mucin secretion related disease such as cancer.