Diabetes mellitus (DM) is a cause of male infertility, but the differences of adverse reproductive parameters have never been elucidated. This study aimed to distinguish between type 1 and type 2 diabetes (T1DM and T2DM) on reproductive parameters using male mice treated with multiple-low doses of streptozotocin (MLD-STZ, T1DM) and high-fat diet with streptozotocin (HFD-STZ, T2DM) for 36 and 72 days. In T1DM, mice were intraperitoneally injected with STZ (40 mg/kg body weight) for 5 days. For T2DM, mice received HFD for 14 days and induced by STZ (85 mg/kg body weight) with fed by HFD continuously. Male reproductive parameters, indicating sperm quality and histology were evaluated. The results showed that reproductive organ weights were significantly decreased in DM mice, but seminal vesicle plus prostate gland of T1DM was lower than that of T2DM. The increases of blood glucose levels, sperm abnormality, and incomplete sperm DNA packaging were observed in T1DM and T2DM groups. Sperm concentration and normal sperm morphology were significantly decreased in T1DM compared to those of T2DM. Seminiferous histopathology of DM animals was classified into 7 types. Morphometry of seminiferous tubule and penis was decreased whereas tunica albuginea thickness and penile collagen fibers were increased in DM mice. Round cells were abundantly found in the epididymal lumens of DM mice. In conclusion, the adverse reproductive parameters of T1DM were more present than T2DM.