Recently, number of dogs have been treated with cell therapy showing variable effect among the treated dogs. Dog Leukocyte Antigen (DLA) have many genes associated with immune system. Therefore, matching of DLA gene is considered an important factor for cell therapy. The aim of the this study was to investigate dog leukocyte antigen(DLA) class II alleles and haplotypes. Sequence-based genotyping of the polymophism region exon2 from DQA,DQB,DRB class II loci were performed the normal 5 dog(Beagle) in 2 pedigree(parent2,sibling3). The allele names determined according to the universal nomenclature found in IPD-MHC sequence data base. DLA- DQA : 014012, DQB : 04201, DRB : 02002 were used as a reference gene and alignment was performed using seqman software. We identified haplotype (DLA-DQA*00401/DQB*02301/DRB*04801) in first pedigree and (DLA-DQA*00101/DQB00201/DRB*00101) in second pedigree. Many Single nucleotide polymorphism(SNPs) to tag haplotype compared to the reference gene. In these SNPs DLA-DRB has the highest SNP(1 pedigree:22(60%), 2 pedigree :28(67%)). These results suggest that DRB is the most highly polymorphic in classII loci. This study serve as a reference for donor and recipient matching in the canine model for investigated immunomodulatory function of DLA-identical cell therapy.